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Volume 41 Issue 5
May  2025
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Article Navigation >  Journal of Clinical Hepatology  > 2025  >  41(5): 862-871

Features of intestinal flora in patients with nonalcoholic fatty liver disease and Helicobacter pylori infection

DOI: 10.12449/JCH250511

  • Department of Gastroenterology，The Second Affiliated Hospital of Henan University of Science and Technology，Luoyang，Henan 471000，China

Research funding:

Luoyang Science and Technology Plan Project (2022063Y)

More Information
  • Corresponding author: LI Renping， 1823131236@qq.com （ORCID： 0009-0005-3109-3013）
  • Received Date: 2024-10-10
  • Accepted Date: 2024-10-29
  • Published Date: 2025-05-25
    Abstract
  •   Objective  To investigate the features and mechanism of action of intestinal flora in patients with nonalcoholic fatty liver disease （NAFLD） and Helicobacter pylori （HP） infection by comparing the changes in intestinal flora between the healthy population， the patients with HP infection， the patients with NAFLD， and the patients with NAFLD and HP infection.  Methods  This study was conducted among the 19 patients with NAFLD （NAFLD group）， 19 patients with HP infection （HP group）， and 19 patients with NAFLD and HP infection （NAFLD+HP group） who were admitted to The Second Affiliated Hospital of Henan University of Science and Technology from March 1， 2023 to April 30， 2024， and 20 individuals undergoing physical examination were enrolled as control group. Fecal samples were collected， total DNA was extracted for PCR amplification， and 16S rDNA sequencing was performed to compare the features of intestinal flora between the four groups. An analysis of variance was used for comparison of continuous data between multiple groups， and the chi-square test was used for comparison of categorical data between multiple groups. The Mann-Whitney U test or the Kruskal-Wallis H test was used for comparison of the species in intestinal flora.  Results  The NAFLD+HP group showed a tendency of reduction in flora abundance compared with the other three groups. There was a significant difference in flora distribution between the NAFLD+HP group and the NAFLD group and between the NAFLD group and the control group （P<0.05）. At the phylum level， the top three species in the NAFLD+HP group were Firmicutes （59.94%）， Proteobacteria （17.00%）， and Actinobacteria （14.75%）， with an increase in the proportion of Proteobacteria and a reduction in the proportion of Actinobacteria compared with the other three groups. At the genus level， the top five dominant bacteria in the NAFLD+HP group were Bifidobacterium， Streptococcus， Escherichia-Shigella， Agathobacter， and Ruminococcus gnavus_group. Compared with the NAFLD group， the NAFLD+HP group had increases in the abundance of Streptococcus， Veillonella， and Rothia and reductions in the abundance of Dialister and Ruminococcus toraues_group. Compared with the HP group， the NAFLD+HP group had reductions in the abundance of Collinsella， Subdoligranulum， Catenibacterium， and Porphyromonas and increases in the abundance of Citrobacter and Olsenella （all P<0.05）.  Conclusion  Patients with NAFLD and HP infection have changed in intestinal flora. These flora may be the intestinal microecological factors for HP infection in promoting the development and progression of NAFLD.

     

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    • References(23)
  • [1]
    FANG J, YU CH, LI XJ, et al. Gut dysbiosis in nonalcoholic fatty liver disease: Pathogenesis, diagnosis, and therapeutic implications[J]. Front Cell Infect Microbiol, 2022, 12: 997018. DOI: 10.3389/fcimb.2022.997018.
    [2]
    ZHANG YB, GUO GY, ZHENG CH, et al. Research progress in association between Helicobacter pylori and metabolic syndrome and its effect on occurrence and development of metabolic syndrome[J]. J Jilin Univ(Med Edit), 2024, 50( 6): 1757- 1762. DOI: 10.13481/j.1671-587X.20240631.

    张艳彬, 郭光业, 郑彩华, 等. 幽门螺杆菌与代谢综合征关联性及其对代谢综合征发生发展影响的研究进展[J]. 吉林大学学报(医学版), 2024, 50( 6): 1757- 1762. DOI: 10.13481/j.1671-587X.20240631.
    [3]
    ABO-AMER YE, SABAL A, AHMED R, et al. Relationship between Helicobacter pylori infection and nonalcoholic fatty liver disease(NAFLD) in a developing country: A cross-sectional study[J]. Diabetes Metab Syndr Obes, 2020, 13: 619- 625. DOI: 10.2147/DMSO.S237866.
    [4]
    OKUSHIN K, TSUTSUMI T, IKEUCHI K, et al. Helicobacter pylori infection and liver diseases: Epidemiology and insights into pathogenesis[J]. World J Gastroenterol, 2018, 24( 32): 3617- 3625. DOI: 10.3748/wjg.v24.i32.3617.
    [5]
    Chinese Society of Hepatology, Chinese Medical Association. Guidelines for the prevention and treatment of metabolic dysfunction-associated(non-alcoholic) fatty liver disease(Version 2024)[J]. J Prac Hepatol, 2024, 27( 4): 494- 510.

    中华医学会肝病学分会. 代谢相关(非酒精性)脂肪性肝病防治指南(2024年版)[J]. 实用肝脏病杂志, 2024, 27( 4): 494- 510.
    [6]
    KONING M, HERREMA H, NIEUWDORP M, et al. Targeting nonalcoholic fatty liver disease via gut microbiome-centered therapies[J]. Gut Microbes, 2023, 15( 1): 2226922. DOI: 10.1080/19490976.2023.2226922.
    [7]
    ZHANG DY, WANG Q, BAI FH. Bidirectional relationship between Helicobacter pylori infection and nonalcoholic fatty liver disease: Insights from a comprehensive meta-analysis[J]. Front Nutr, 2024, 11: 1410543. DOI: 10.3389/fnut.2024.1410543.
    [8]
    MANTOVANI A, LANDO MG, BORELLA N, et al. Relationship between Helicobacter pylori infection and risk of metabolic dysfunction-associated steatotic liver disease: An updated meta-analysis[J]. Liver Int, 2024, 44( 7): 1513- 1525. DOI: 10.1111/liv.15925.
    [9]
    THURSBY E, JUGE N. Introduction to the human gut microbiota[J]. Biochem J, 2017, 474( 11): 1823- 1836. DOI: 10.1042/BCJ20160510.
    [10]
    LEVY M, KOLODZIEJCZYK AA, THAISS CA, et al. Dysbiosis and the immune system[J]. Nat Rev Immunol, 2017, 17( 4): 219- 232. DOI: 10.1038/nri.2017.7.
    [11]
    BOURSIER J, MUELLER O, BARRET M, et al. The severity of nonalcoholic fatty liver disease is associated with gut dysbiosis and shift in the metabolic function of the gut microbiota[J]. Hepatology, 2016, 63( 3): 764- 775. DOI: 10.1002/hep.28356.
    [12]
    MAVILIA-SCRANTON MG, WU GY, DHARAN M. Impact of Helicobacter pylori infection on the pathogenesis and management of nonalcoholic fatty liver disease[J]. J Clin Transl Hepatol, 2023, 11( 3): 670- 674. DOI: 10.14218/JCTH.2022.00362.
    [13]
    GAO JJ, ZHANG Y, GERHARD M, et al. Association between gut microbiota and Helicobacter pylori-related gastric lesions in a high-risk population of gastric cancer[J]. Front Cell Infect Microbiol, 2018, 8: 202. DOI: 10.3389/fcimb.2018.00202.
    [14]
    NABAVI-RAD A, SADEGHI A, ASADZADEH AGHDAEI H, et al. The double-edged sword of probiotic supplementation on gut microbiota structure in Helicobacter pylori management[J]. Gut Microbes, 2022, 14( 1): 2108655. DOI: 10.1080/19490976.2022.2108655.
    [15]
    DU LJ, CHEN BR, CHENG FL, et al. Effects of Helicobacter pylori therapy on gut microbiota: A systematic review and meta-analysis[J]. Dig Dis, 2024, 42( 1): 102- 112. DOI: 10.1159/000527047.
    [16]
    WANG YH, HUANG Y. Effect of Lactobacillus acidophilus and Bifidobacterium bifidum supplementation to standard triple therapy on Helicobacter pylori eradication and dynamic changes in intestinal flora[J]. World J Microbiol Biotechnol, 2014, 30( 3): 847- 853. DOI: 10.1007/s11274-013-1490-2.
    [17]
    WEISS G, FORSTER S, IRVING A, et al. Helicobacter pylori VacA suppresses Lactobacillus acidophilus-induced interferon beta signaling in macrophages via alterations in the endocytic pathway[J]. mBio, 2013, 4( 3): e00609-12. DOI: 10.1128/mBio.00609-12.
    [18]
    AHN SB, JUN DW, KANG BK, et al. Randomized, double-blind, placebo-controlled study of a multispecies probiotic mixture in nonalcoholic fatty liver disease[J]. Sci Rep, 2019, 9( 1): 5688. DOI: 10.1038/s41598-019-42059-3.
    [19]
    den BESTEN G, van EUNEN K, GROEN AK, et al. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism[J]. J Lipid Res, 2013, 54( 9): 2325- 2340. DOI: 10.1194/jlr.R036012.
    [20]
    YOON SJ, YU JS, MIN BH, et al. Bifidobacterium-derived short-chain fatty acids and indole compounds attenuate nonalcoholic fatty liver disease by modulating gut-liver axis[J]. Front Microbiol, 2023, 14: 1129904. DOI: 10.3389/fmicb.2023.1129904.
    [21]
    BLAAK EE, CANFORA EE, THEIS S, et al. Short chain fatty acids in human gut and metabolic health[J]. Benef Microbes, 2020, 11( 5): 411- 455. DOI: 10.3920/BM2020.0057.
    [22]
    MBAYE B, MAGDY WASFY R, BORENTAIN P, et al. Increased fecal ethanol and enriched ethanol-producing gut bacteria Limosilactobacillus fermentum, Enterocloster bolteae, Mediterraneibacter gnavus and Streptococcus mutans in nonalcoholic steatohepatitis[J]. Front Cell Infect Microbiol, 2023, 13: 1279354. DOI: 10.3389/fcimb.2023.1279354.
    [23]
    CROST EH, COLETTO E, BELL A, et al. Ruminococcus gnavus: Friend or foe for human health[J]. FEMS Microbiol Rev, 2023, 47( 2): fuad014. DOI: 10.1093/femsre/fuad014.
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